SPOROTRICHUM THERMOPHILE CULTURE EXTRACT-MEDIATED GREEN SYNTHESIS OF SILVER NANOPARTICLES: ECO-FRIENDLY FUNCTIONAL GROUP TRANSFORMATION AND ANTI-BACTERIAL STUDY

Author (s) : T.Gnanasambandan, S.Rafiahamed, K.Sasikala, V.Velvizhi

---

Abstract :
Thermophilic mould Sporotrichum thermophile BJTLRMDU7 played the role in greener synthesis of the silver nanoparticles (AgNPs) extracellularly using silver nitrate. The change in colour from transparent to dark brown primarily indicated the formation of AgNPs due to reduction of Ag (I) ions to Ag by the fungal culture extract. Nanoparticles synthetic process was optimized using “one variable at a time” approach. Sucrose containing medium having pH 8.0 supported the synthesis of AgNPs by the mould at 45 ​°C. Furthermore, presence of light significantly accelerated the formation of silver nanoparticles. AgNPs were characterized by various techniques like UV–vis & FT-IR spectroscopy, dynamic light scattering (DLS) and X-ray diffraction (XRD). Appearance of a band at 426 ​nm in UV–vis spectrum revealed the reduction of Ag(I) ions to Ag (0) by mould’s culture filtrate. DLS data showed that AgNPs bear an average size of 70 ​nm, while XRD data revealed particle size of 40 ​nm. The application of AgNPs with their functional group transformation has successfully reduced p-nitrophenol into p-aminophenol in an eco-friendly manner in the presence of light and NaBH₄. Further, the synthesized AgNPs showed anti-bacterial potential against Gram positive and Gram negative bacteria.

Keywords : Silver nanoparticles,Greener approach,Sporotrichum thermophile,Anti-bacterial activity,Organic synthesis, Spectroscopy

Conference Date : 30/08/2019

Pages : 361

Paper ID : 19352