Molecular Characterization of CTX-M β-Lactamase Producing Enterobacteriaceae from Urine Samples in Salem

Author (s) : M.Prithya

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Abstract :
The emergence of Extended-spectrum beta lactamase (ESBL) producing bacteria, particularly Escherichia coli and Klebsiella pneumoniae, is now a critical concern for the development of therapies against bacterial infection. Urinary Tract Infection (UTI) remains the most common bacterial infection in the human population. This observational study was conducted at the SKS Hospital, Salem, from December 2013 to February 2014. Totally 70 urine specimens were used for the study and of which only 30 isolates were found out to be belonging Enterobacteriaceae group of E. coli, K. Pneumoniae and P.aeruginosa. These 30 isolates were subjected for the antibiotic sensitivity test using Kirby- Bauer method and 22 (73.3%) isolates were found to be ESBL, which were confirmed by using the Double Disc Synergy Test (DDST). ESBL production was detected in 16 (73%) E. coli, 2 (9%) K. Pneumoniae and 4 (18%) P.aeruginosa. ESBL producing organisms showed maximum resistance to Ampicillin, Cloxacillin, Cefuroxime, Lomefloxacin, and Ciprofloxacin while minimum resistance was seen with Imipenem.Proper antibiotic policy and appropriate guidelines to prescribe antibiotics are the routes to prevent dissemination of Multidrug Resistant Organisms. The goal of this study was to detect the ESBL producing Enterobacteriaceae from urine and detect the drug resistance CTX-M gene by PCR method.

Keywords : Molecular Characterization

Conference Date : 17/09/2021

Pages : 302

Paper ID : IESMDT300