ISSN : 2349-6657

ISOLATION AND CHARACTERIZATION OF AGROBACTERIUM RHIZOGENES FROM THE ROOT NODULES OF SOME LEGUMINOUS PLANTS

Dr.K.Karthika and T.Bhuvana



Using yeast extract mannitol agar (YEMA), 57 colonies of Agrobacterium species were found in the root nodules of five distinct leguminous plants, including Pisum sativum, Sesbania rostrata, Vigna mungo, V. radita, and V. ungiculata. Agrobacterium species were present in all of the isolated colonies. The morphological, biochemical, cultural, and pathogenicity tests allowed for the characterization of the isolated bacteria and the identification of it as Agrobacterium rhizogenes. The biochemical properties of the agrobacterial isolates, including exopolysaccharide, glycogen, total protein, total free amino acids, and total lipids, were estimated. The biochemical components of the agrobacteria from various leguminous plants varied greatly.

Leguminous plants % Agrobacterium rhizogenes % Characterization % Biochemical constituents.

30/08/2019

104

19102

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