IN VITRO ANTIBACTERIAL STUDY OF PEEL, PULP AND SEED KERNEL EXTRACTS OF MANGIFRA INDICA AGAINST SELECTED BACTERIAL PATHOGENIC STRAINS.

Author (s) : S.Abinaya & S.Shenbagavalli

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Abstract :
Mango (Mangifera indica) is an important tropical fruit worldwide due to its flavor and health promoting activity. Mangoes available in dry zone area of Sri Lanka have high consumer demand due to unique flavor. The main aim of this study was to investigate antibacterial activity of the peel, pulp and seed kernel of Mango. In the present study, three mango cultivars, namely ‘Willard’, ‘Karuthacolomban’ and ‘Vellaicolomban’ were collected from Jaffna district in Sri Lanka during April to July, 2015 to carry out the extraction processes. Ethyl acetate extracts of flesh, peel and seed kernel of three mango varieties were tested for antibacterial activity using agar well diffusion method at 10 mg/mL against Escherichia coli [ATCC 25922], Staphylococcus aureus [ATCC 25923] and Bacillus subtilis [MTCC 121]. The results revealed that seed kernel of ‘Karuthacolomban’ and ‘Willard’ showed antibacterial activity against E. coli. Seed kernel extracts of ‘Karuthacolomban’, ‘Willard’ and ‘Vellaicolomban’ showed the antibacterial activity against S.aureus and B.subtilis. None of peel and pulp extracts inhibited the growth of E.coli, S.aureus and B.subtilis. The active extracts were next tested at 1.25, 2.50, 5 mg/mL. At 5 mg/mL concentration, seed kernel of ‘Karuthacolomban’ showed the antibacterial activity against E. coli and S.aureus. The above observation suggests that ethyl acetate extract of “Karuthacolomban’ seed kernel shows the highest antibacterial activity against all the three tested microorganisms. These results demonstrate that seed kernel of mango extracts are capable of producing antibacterial substance against selected pathogenic bacteria.

Keywords : Mango, peel, seed, pulp, Antibacterial activity

Conference Date : 30/08/2019

Pages : 266

Paper ID : 19257