IN VITRO ANALYSIS OF SECONDARY METABOLISM AND ANTAGONIST ACTIVITY OF ENDOPHYTIC FUNGI

Author (s) : S.Anbumalar & S.Ambiga

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Abstract :
Endophytic fungi, residing within the internal tissues of plants, have been recognized as prolific sources of bioactive secondary metabolites with various applications in medicine, agriculture, and industry. In this study, we conducted an in vitro analysis of the secondary metabolism and antagonist activity of endophytic fungi isolated from diverse plant species.The research involved the collection of plant samples from different ecological niches, followed by surface sterilization and isolation of endophytic fungi. A total of [insert number] endophytic fungal strains were successfully isolated and characterized through morphological and molecular techniques.To investigate their secondary metabolism, the endophytic fungi were cultured on different nutrient media, and the extraction of secondary metabolites was performed using suitable solvents. High-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses were employed to identify and quantify the metabolites produced. Furthermore, we evaluated the antagonist activity of the endophytic fungi against various pathogenic microorganisms. In vitro assays were conducted to assess the inhibitory effects of fungal extracts on the growth and viability of selected bacterial and fungal pathogens. The results revealed significant antagonistic potential exhibited by several endophytic fungal strains against various test pathogens.This study sheds light on the immense potential of endophytic fungi as valuable resources for the production of bioactive compounds and the development of novel antimicrobial agents. The findings contribute to our understanding of the complex interactions between endophytic fungi and their host plants and highlight the importance of exploring these microorganisms for biotechnological applications.

Keywords : endophytic fungi, secondary metabolism, antagonist activity, bioactive compounds, in vitro analysis.

Conference Date : 30/08/2019

Pages : 167

Paper ID : 19165