GREEN SYNTHESIS OF SILVER NANOPARTICLES USING CASSIA AURICULATA FLOWER EXTRACTS: ANTIBACTERIAL ACTIVITY AND CHARACTERIZATION ANALYSIS

Author (s) : S.Chandralekha & S.Ajithadevi

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Abstract :
Silver nanoparticles (AgNPs) have attracted considerable attention due to their diverse applications, particularly in the field of medicine and healthcare. The synthesis of AgNPs using green and sustainable approaches has gained prominence as an eco-friendly alternative to conventional chemical methods. In this study, we report the green synthesis of silver nanoparticles using Cassia auriculata flower extracts and investigate their antibacterial activity against pathogenic microorganisms. Additionally, the synthesized AgNPs are characterized to understand their physicochemical properties.The Cassia auriculata flower extract was obtained through a simple and cost-effective aqueous extraction process, which acts as both a reducing and stabilizing agent for AgNP synthesis. The reduction of silver ions to AgNPs was confirmed by the change in color of the reaction mixture from pale yellow to dark brown, indicating the formation of nanoparticles. The characterization of the synthesized AgNPs was carried out using various techniques such as UV-visible spectroscopy, which revealed a characteristic surface plasmon resonance (SPR) peak around 400-450 nm, confirming the presence of AgNPs. Fourier-transform infrared spectroscopy (FTIR) analysis was performed to identify the functional groups responsible for the reduction and stabilization of AgNPs by the flower extract. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were utilized to determine the size and morphology of the nanoparticles, which were found to be mostly spherical and ranging from 10 to 50 nm in size.The antibacterial activity of the synthesized AgNPs was evaluated against common pathogenic bacteria, including Escherichia coli and Staphylococcus aureus, using standard agar diffusion assays. The results demonstrated significant inhibition zones around the AgNP-treated discs, indicating their potent antibacterial activity against both gram-negative and gram-positive bacteria.The green synthesis of AgNPs using Cassia auriculata flower extracts offers an environmentally friendly and biocompatible route for producing nanoparticles with notable antibacterial properties.

Keywords : Silver nanoparticles,characterization, biocompatible.

Conference Date : 30/08/2019

Pages : 177

Paper ID : 19172