ENHANCED BACTERICIDAL ACTIVITY AND OSTEOBLAST CELL ABUNDANCE OF DUAL METAL IONS CO -SUBSTITUTED HYDROXYAPATITE – POLY(METHYL METHACRYLATE) BIOCOMPOSITE IMPLANT FOR ORTHOPEADIC APPLICATIONS

Author (s) : S.JEEVA and D.DHANALAKSHMI

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Abstract :
Poly (methyl methacrylate) PMMA bone cement though unbeaten in the area of bone tissue engineering undergo from a lack of bio-action, not actually join together with contiguous bone tissues. Bioactive obsession is anticipated to increase PMMA- based cement performance because of the usual interlacing and coupling with bone tissues, which can recover the enhancive potential of the fabric in uses for instance, vertebroplasty. The improvement of living bone tissue assembles with physical and biological efficient resemblances to usual bone tissue are the foremost challenge in orthopedics tissue engineering. To conquer these challenges, cerium- copper minerals co- substituted hydroxyapatite (Ce- Mg-HA)/ poly (methyl methacrylate) (PMMA) (Ce- Mg -HA/ PMMA) bio-composite have been fabricated through solvent vanishing technique. The Fourier transform infrared (FT- IR), X-Ray diffraction (XRD), scanning electron microscope- energy dispersive X-Ray spectroscopy (SEM- EDX) and transmission electron microscopy- Selected area diffraction (TEM- SAD) study established the existence of Ce- Mg -HA in the PMMA matrix. Moreover, the as-fabricated biocomposite demonstrated strong bactericidal activity on gram-negative bacteria (E.coli) and gram-positive bacteria (Klebsiella ) which may be owing to the action of release of Ce and Cu ions on the exterior that chunks the carrying channels of the cell leading to the cell bereavement. The biological investigations recommended that the as-fabricated bio-composite promotes the propagation, ALP action, calcium deposition and collagen formation of osteoblast cells co- colonization on the Ce- Mg -HA/PMMA bio-composite. Therefore, the as-fabricated bio-composite confirm outstanding versatile properties that could be employing in bone tissue engineering.

Keywords : Bio-composite; Biocompatibility; antibacterial activity; Osteogenic; osseointegration; PMMA

Conference Date : 30/08/2019

Pages : 40

Paper ID : 19038