DEVELOPMENT OF ENZYMES IMMOBILIZED POLY ( METHYL METHACRYLATE) SURFACES FOR BIOFLIM INHIBITION OF ESKAPE PATHOGENS

Author (s) : Dr.V.K.EVANJALENE, M.GOMATHI, S.ABINAYA

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Abstract :
The development of enzymes immobilized on poly (methyl methacrylate) (PMMA) surfaces for biofilm inhibition of ESKAPE pathogens is a promising approach to combat the escalating threat of antibiotic-resistant infections. ESKAPE pathogens encompass a group of multidrug-resistant bacteria that are particularly challenging to treat and are responsible for a significant portion of hospital-acquired infections. Biofilms formed by these pathogens further exacerbate the problem by providing protective niches that render antibiotics ineffective. Enzyme-based biofilm inhibition offers a novel and targeted strategy to disrupt biofilm formation and improve treatment outcomes. This study focuses on the immobilization of potent anti-biofilm enzymes onto PMMA surfaces to create biomaterials with enhanced antimicrobial properties. The immobilization process and characterization techniques will be investigated to ensure enzyme stability and bioactivity. The developed enzyme-immobilized PMMA surfaces will be evaluated for their ability to inhibit ESKAPE pathogen biofilms through in vitro and ex vivo studies. The results of this research hold the potential to pave the way for innovative antimicrobial biomaterials that can effectively combat ESKAPE pathogen infections and reduce the burden of antibiotic resistance in healthcare settings.

Keywords : Biofilm, pathogens, PMMA, antimicrobial, biomaterials

Conference Date : 13/11/2020

Pages : 205

Paper ID : 20205