BIOLOGICAL CONTROL OF BACTERIAL BLIGHT DISEASE OF POMEGRANATE USING SILVER NANOPARTICLES

Author (s) : R.PRIYA & S.RAMYA

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Abstract :
Bacterial blight, caused by Xanthomonas axonopodis pv. punicae, is a devastating disease that significantly affects pomegranate cultivation worldwide. Conventional methods of disease management have often led to environmental concerns and development of resistant strains, necessitating the exploration of eco-friendly alternatives. Silver nanoparticles (AgNPs) have emerged as a promising tool for the biological control of plant diseases due to their unique antimicrobial properties.   This study investigates the efficacy of silver nanoparticles as a potential biocontrol agent against bacterial blight disease of pomegranate. Green synthesis of AgNPs was achieved using an aqueous extract of plant material rich in phytochemicals, ensuring an environmentally benign approach. The synthesized AgNPs were characterized using various analytical techniques such as UV-Vis spectroscopy, dynamic light scattering, and transmission electron microscopy to determine their size, shape, and stability.   In vitro experiments demonstrated that the synthesized AgNPs exhibited significant antimicrobial activity against Xanthomonas axonopodis pv. punicae. The minimum inhibitory concentration (MIC) was determined, and the results revealed the potent bactericidal effect of AgNPs at low concentrations. Additionally, AgNPs were non-toxic to pomegranate plant cells, ensuring the safety of their application as a potential biocontrol agent.   Greenhouse trials were conducted to evaluate the in vivo efficacy of AgNPs against bacterial blight disease in pomegranate plants. Foliar spray application of AgNPs effectively reduced disease severity and promoted plant growth compared to the control group. Furthermore, the application of AgNPs did not adversely affect the beneficial microbial population in the rhizosphere, emphasizing their eco-friendly nature.   This research establishes the feasibility of using silver nanoparticles as an efficient and environmentally sustainable alternative for controlling bacterial blight disease in pomegranate cultivation. The findings pave the way for further exploration of AgNPs in integrated disease management strategies, aiming to improve pomegranate production while minimizing the environmental impact of conventional pesticides. However, further research on large-scale field trials and potential long-term effects is essential to fully comprehend the practical application of AgNPs in commercial pomegranate orchards.

Keywords : Xanthomonas axonopodis, antimicrobial properties.

Conference Date : 30/08/2019

Pages : 169-170

Paper ID : 19167