BIOLOGICAL APPLICATIONS OF SYNTHESIZED ZNO NANOPARTICLES USING PLEUROTUS DJAMOR AGAINST MOSQUITO LARVICIDAL, HISTOPATHOLOGY, ANTIBACTERIAL, ANTIOXIDANT AND ANTICANCER EFFECT

Author (s) : Dr.M.MEENAMBAL, J.SEETHALAKSHMI

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Abstract :
The current work focused on the biosynthesis, characterisation, and biological applications of zinc oxide nanoparticles (ZnONPs) from Pleurotus djamor, including larvicidal, histopathological, antibacterial, antioxidant, and anticancer properties. The synthesized NPs were examined under a microscope and analyzed using spectral data. A 350 nm peak UV-Visible spectrophotometer was then used to confirm the results. With the lowest LC50 and LC90 values (10.1, 25.6 and 14.4, 31.7 mg/l, respectively) after 24 h of treatment, the ZnONPs had significant antioxidant property (DPPH, H2O2, and ABTS? radical assay) and shown good larval toxicity against Ae. aegypti and Cx. quinquefasciatus (IVth instar larvae). The treated larvae showed morphological alterations, including damaged cuticle layers and the anal papillae area. With regard to the antibacterial assay, C. diphteriae had the highest growth inhibition zone (28.6 0.3 mm), followed by P. fluorescens (27 0.5 mm), and S. aureus (26.6 1.5 mm). Even at low doses, ZnONPs showed strong cytotoxic effects against the A549 lung cancer cells in the in vitro cytotoxicity experiment (LC50 values 42.26 lg/ml). The study's overall conclusions point to P. djamor's capacity for ZnONP biosynthesis and the possibility that it could serve as a substitute biological agent for upcoming therapeutic uses in medical fields.

Keywords : Pleurotus djamor, larvicidal, histopathological, antibacterial, antioxidant, and anticancer properties

Conference Date : 30/08/2019

Pages : 106

Paper ID : 19104