A STUDY ON THE SYNTHESIS OF SILVER NANOPARTICLES AND ANTIBACTERIAL ACTIVITY OF SENNA HIRSUTA

Author (s) : Ms. S.Ajithadevi

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Abstract :
Silver nanoparticles (AgNPs) have gained significant attention in various fields due to their unique properties and applications, particularly in nanomedicine and antibacterial therapies. In this study, we focused on the synthesis of silver nanoparticles using a green and eco-friendly approach and evaluated their antibacterial potential using Senna hirsuta leaf extract. The synthesis of silver nanoparticles was achieved through the reduction of silver ions (Ag+) using Senna hirsuta leaf extract as the reducing and capping agent. The process was simple, cost-effective, and environmentally benign, making it an attractive alternative to conventional chemical methods. The synthesized AgNPs were characterized using various analytical techniques, including UV-Visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR), to determine their size, shape, crystallinity, and surface functional groups. The antibacterial activity of the synthesized AgNPs was assessed against a panel of pathogenic bacteria, including Gram-positive and Gram-negative strains, using well-established agar diffusion and broth microdilution assays. Senna hirsuta leaf extract and commercially available antibiotics were used as controls for comparison. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined to assess the effectiveness of the AgNPs against the tested bacterial strains. The results demonstrated that the green-synthesized silver nanoparticles exhibited remarkable antibacterial activity against a broad spectrum of pathogenic bacteria. The nanoparticles showed a dose-dependent inhibition of bacterial growth, with significantly lower MIC and MBC values compared to the Senna hirsuta leaf extract alone. Moreover, the AgNPs displayed superior antibacterial efficacy compared to conventional antibiotics against some bacterial strains. The findings of this study highlight the potential of Senna hirsuta-mediated silver nanoparticles as effective and eco-friendly antibacterial agents. The green synthesis method offers an alternative route for the production of antimicrobial nanoparticles, reducing the dependence on hazardous chemicals and promoting sustainable nanotechnology. Further studies are warranted to explore the underlying mechanisms of the antibacterial activity and to assess the nanoparticles' biocompatibility for potential biomedical applications.

Keywords : Silver nanoparticles, antibacterial activity

Conference Date : 17/09/2021

Pages : 119

Paper ID : IESMDT117