THE GROWTH OSCILLATOR AND PLANT STOMATA: AN OPEN AND SHUT CASE

Author (s) : C.Maheshwari & I. Krishnaveni M. Indumathi & M. Meenambal

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Abstract :
Since Darwin’s “Power of Movement in Plants” the precise mechanism of oscillatory plant growth remains elusive. Hence the search continues for the hypothetical growth oscillator that regulates a huge range of growth phenomena ranging from circumnutation to pollen tube tip growth and stomatal movements. Oscillators are essentially simple devices with few components. A universal growth oscillator with only four major components became apparent recently with the discovery of a missing component, notably arabinogalactan glycoproteins (AGPs) that store dynamic Ca²⁺ at the cell surface. Demonstrably, auxin-activated proton pumps, AGPs, Ca²⁺ channels, and auxin efflux “PIN” proteins, embedded in the plasma membrane, combine to generate cytosolic Ca²⁺ oscillations that ultimately regulate oscillatory growth: Hechtian adhesion of the plasma membrane to the cell wall and auxin-activated proton pumps trigger the release of dynamic Ca²⁺ stored in periplasmic AGP monolayers. These four major components represent a molecular PINball machine a strong visual metaphor that also recognises auxin efflux “PIN” proteins as an essential component. Proton “pinballs” dissociate Ca²⁺ ions bound by paired glucuronic acid residues of AGP glycomodules, hence reassessing the role of proton pumps. It shifts the prevalent paradigm away from the recalcitrant “acid growth” theory that proposes direct action on cell wall properties, with an alternative explanation that connects proton pumps to Ca²⁺ signalling with dynamic Ca²⁺ storage by AGPs, auxin transport by auxin-efflux PIN proteins and Ca²⁺ channels. The extensive Ca²⁺ signalling literature of plants ignores arabinogalactan proteins (AGPs). Such scepticism leads us to reconsider the validity of the universal growth oscillator proposed here with some exceptions that involve marine plants and perhaps the most complex stress test, stomatal regulation.

Keywords : growth oscillator; calcium signalling; arabinogalactan protein; pinball machine; stomata

Conference Date : 30/08/2019

Pages : 270

Paper ID : 19261