ANTHER RESPONSE IN INDICA RICE IS AFFECTED BY THE GROWING SEASON AND INORGANIC NITROGEN IN THE PRE-CULTURE MEDIUM

Author (s) : Ms. K. GAYATHRI, Dr.G. SAKTHIVEL & R. ANUPRIYA

---

Abstract :
Success of anther culture in rice is affected by genetic and non-genetic factors. This study investigated the effect of non-genetic factors on in vitro anther response in indica rice. In Sri Lanka, Yala and Maha seasons provide vastly different climate conditions for rice cultivation. Six anther culture responsive rice varieties (At 303, Bg 358, Bw 272-6B, Dahanala, Herath Banda, Dikwee) were raised in a Randomized Complete Block Design (RCBD) at the Regional Rice Research and Development Centre, Bombuwela, over the two rice growing seasons Maha (2013/14) and Yala (2014). From each variety, booting stage panicles were harvested, cold pre-treated and excised anthers were cultured on N6 medium for callus induction. Anther response varied between varieties and for each variety a significantly higher response (p≤0.05) was observed in anthers cultured from plants grown in the Yala season than in the Maha season. The highest improvement was seen in variety Dahanala in which the anther response increased from 9.97% in Maha to 16.87% in Yala. Using rice variety At 303 the effect of short duration (3 day) pre-culture of anthers on N6 medium having different inorganic nitrogen sources, NO3 - and/or NH4 + on anther response was examined. Callus induction frequency increased significantly (p≤0.05) with complete withdrawal of (NH4)2SO4 from the standard N6 medium (10.38%) compared to the control (3.17%). Withdrawal of half the concentration of (NH4)2SO4 from the standard N6 medium also recorded a fairly high callus induction response (5.39%). Thus, abiotic stresses applied to donor plants, as well as on anthers prior to culture on callus induction media, significantly improve anther response in indica rice.

Keywords : anther culture, indica rice, growing season, Nitrogen, pre-treatment

Conference Date : 17/09/2021

Pages : 193

Paper ID : IESMDT191