ANTIMICROBIAL ANALYSIS OF EDIBLE EXTRACTS AGAINST WOUND INFECTION

Author (s) : Dr.S.Ambiga

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Abstract :
Wound infections represent a significant healthcare challenge, necessitating the development of effective and safe antimicrobial agents. In recent years, interest has grown in exploring natural alternatives, such as edible plant extracts, as potential sources of antimicrobial compounds. This study aimed to investigate the antimicrobial properties of various edible extracts against wound infection-causing pathogens. A comprehensive screening of edible plant extracts was conducted, involving the extraction of bioactive compounds using different solvent systems. The extracts were tested against a panel of clinically relevant wound pathogens, including both Gram-positive (e.g., Staphylococcus aureus) and Gram-negative (e.g., Pseudomonas aeruginosa) bacteria. The antimicrobial activity was assessed using well-established standard microbiological methods, including disc diffusion and broth microdilution assays. Results revealed varying degrees of antimicrobial activity among the tested extracts. Several extracts exhibited potent inhibitory effects against the tested pathogens, with some even demonstrating broad-spectrum activity against both Gram-positive and Gram-negative bacteria. The most promising extracts were subjected to further characterization using advanced analytical techniques, such as gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC), to identify the specific bioactive compounds responsible for the observed antimicrobial effects. Furthermore, the safety profile of the most effective extracts was evaluated using in vitro cytotoxicity assays with human dermal fibroblasts to ensure their potential for application in wound healing. The extracts showing substantial antimicrobial activity and minimal cytotoxicity were then assessed in an in vivo wound infection model using animal subjects. This study contributes to the growing body of knowledge on edible plant extracts' antimicrobial potential and their possible application as natural remedies for wound infections. The findings offer promising insights into harnessing these extracts as alternative or complementary therapeutic options to conventional antibiotics, potentially reducing the risk of antimicrobial resistance and promoting wound healing. However, further studies are warranted to elucidate the underlying mechanisms of action and to optimize the formulations for potential clinical translation.

Keywords : Antimicrobial properties, Wound infections

Conference Date : 17/09/2021

Pages : 113

Paper ID : IESMDT111