ISOLATION AND IDENTIFICATION OF MICRO-ORGANISM FROM SOIL SAMPLE

Author (s) : Ms.K.GAYATHRI and Mrs.M.GOMATHI

---

Abstract :
The present work deals with the isolation and characterization of microorganisms from agriculture soil. Microorganism is frequently present in soil, manure and decaying Plant tissues which are able to degrade wastes that are correlated with the substrate organic matter. Several bacteria were isolated from soil, using serial dilution method. These bacterial isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Enterobacter aerogenes, Shigella sp., Proteus mirabilis, Bacillus anthracis, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis species which were further identified on the basis of colony morphology, Gram staining, biochemical tests and using selective and differential media. Microorganisms play an important function in biodegradation of solid agriculture waste and also help in the crop production.

Keywords : soil sample, micro organisms, serial dilution, gram stanning, Biochemical test

Conference Date : 17/09/2021

Pages : 78

Paper ID : IESMDT76